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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
WAY-100635 Maleate
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
1092679-51-0
- 规格:
W409167-1ml
英文名:WAY-100635 Maleate
纯度或规格:10mM in DMSO
SPU:W409167
CAS:1092679-51-0
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Easy Way to Clone Genes From a Phage Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97. The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters
Troubleshooting for PCR and multiplex PCR
. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above 4. My PCR product is weak. Is there a way to increase the yield? Gradually decrease the annealing temperature to the lowest possible. Increase
in an ice bucket and let the cells cool. 2. Now, add the DMSO to the cells slowly. This step is accomplished by adding to the resuspended cells an equal volume of DMEM containing 20% fetal calf serum and 20% DMSO. This should be done dropwise
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