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- 文献和实验
- 技术资料
- 库存:
期货
- 规格:
W424492-1ml
中文名:WAY-324046
英文名:WAY-324046
纯度:10mM in DMSO
货号:W424492
Cas号:524055-94-5
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
NaOH 0.025% Bromophenol Blue 0.025% Xylene Cyanol Run gel in 0.6X TEB buffer. Heat denature samples at 94°C for 5 minutes and place them on ice for 3-5 minutes. Load 2.0-4.0µl per sample. Include non-denatured controls.
of 10 but they are good starting point. ligation dilution 1 ul 10x PCR buffer 5 ul DMSO 3 ul dNTPs (10 mM) 3 ul M13F (350 ng/ul) 1 ul SAGE Primer 2 (350 ng/ul) 1 ul ddH2O 36 ul Tag 1 ul Follow the following PCR cycling condition: 94oC
in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min
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