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- 技术资料
- 库存:
现货
- 规格:
I423214-1ml
中文名:IQ 3
英文名:IQ 3
纯度:10mM in DMSO
货号:I423214
Cas号:312538-03-7
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Natural History Museum Protocol for EST sequencing from lamb
- (if the agarose gel in step 4 only shows a weak band, use less than 50µl of water to elute). Direct Purification Buffer: 50mM KCl (=0.37g/100ml) 10mM Tris-HCL (pH 8.8 at 25℃) (=0.12g/100ml) 1.5mM MgCl2 (=0.03g/100ml) 0.1% Triton X-100 (=100µl/100ml
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
1 drop of chloroform and 35µl DMSO (i. e. 7% final) and place at -70oC (no need to snap freeze). (N. b. store chloroform with some anhydrous sodium bicarbonate in the bottom of the bottle, about 1 teaspoon per 100mls.) (3) PCR
Purification of recombinant sBRF M166L
under lac iQ control Kanomycin resistant) Inoculate 6 ml of a saturated overnight culture of the transformed bacteria into 2X 1 Liter of YT Amp (50 ug/ml) (and Kan (30 ug/ml) if using strain JM25) (adding 1-2 drops of antifoam A before autoclaving the YT
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