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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Silicone oil AP 200
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
63148-58-3
- 规格:
S140418-500g/S140418-2.5kg/S140418-100g
| 规格: | S140418-500g | 产品价格: | ¥1300.9 |
|---|---|---|---|
| 规格: | S140418-2.5kg | 产品价格: | ¥4942.9 |
| 规格: | S140418-100g | 产品价格: | ¥358.9 |
英文名:Silicone oil AP 200
纯度或规格:200 mPa.s, neat(25 °C)
SPU:S140418
CAS:63148-58-3
存储温度:-
运输条件:常规运输
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
of the 1000x tetracyclin stock solution . Inoculate with 25 to 50µl of the glycerol stock. Incubate in 37°C shaker incubator for 6-8 hours. Preparation of host cells from single colony Streak XL1-blue
from Genebridges. Electroporation was performed at 1.35 kV, 25 μF, 200 Ω using an Eppendorf electroporator (2510). Bacteria were incubated in 1 ml LB media at 37°C, 1100 rpm for 1�2 h before plating on agar plates conditioned with the appropriate antibiotic(s
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
) Digested DNA is extracted once with phenol/chloroform, once with chloroform, precipitated with 3 volumes of 100% EtOH, and resuspended in TE at a final concentration of 100-200 µg/ml. c) The following reagents are mixed in 0.5 ml eppendorf tube. water
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