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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
L-α-Phosphatidyl-DL-glycerol ammonium salt from egg yolk lecithin
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
L466189-5mg/L466189-25mg/L466189-100mg
| 规格: | L466189-5mg | 产品价格: | ¥334.9 |
|---|---|---|---|
| 规格: | L466189-25mg | 产品价格: | ¥1244.9 |
| 规格: | L466189-100mg | 产品价格: | ¥4115.9 |
英文名:L-α-Phosphatidyl-DL-glycerol ammonium salt from egg yolk lecithin
纯度或规格:≥99%, solution, 10mg/mL in chloroform: methanol (49:1)
SPU:L466189
CAS:-
存储温度:-20°C储存
运输条件:超低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验via a basic Bligh-Dyer extraction: a) Resuspend cell pellet in 3 ml chloroform/methanol (1:2) & vortex. b) Add 0.8 ml water & vortex. c) Pellet cellular debris by spinning at 3,000 rpm (2000xg) for 5 min. d) Add 1 ml chloroform & vortex. e) Add 1 ml water
diethylenetriaminepentaacetic acid (DTPA) --> Bring volume up to 50 ml with distilled water. Reaction Mixture: solution [stock] vol/tube # samples total vol. 2X buffer - 50 µl X _______ = _______ DTT 1 M 0.2 µl X _______ = _______ DGK membrane
Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells
. 17) Add 50 µl of SMase solution to each reaction tube and allow reaction to proceed for 30 minutes. --> Remember to have a control tube containing no enzyme to determine background. 18) Add 1.5 ml Chloroform/Methanol (2:1) to each tube to stop
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