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- 文献和实验
- 技术资料
- 库存：
现货
- 规格：
G421216-1ml
中文名：Gadodiamide
英文名：Gadodiamide
纯度：10mM in Water
货号：G421216
Cas号：131410-48-5
存储温度：避光,-80℃储存
运输条件：特低温运输
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文献和实验You will need: Freshly made 2M NaOH 3M sodium acetate, pH 4.5 Sterile, distilled water Absolute ethanol 70% ethanol 7 x DNA annealing buffer (280mM Tris.Cl, pH 7.5, 100mM MgCl2, 350mM NaCl) Termination mixes (40mM Tris.Cl, pH 7.5, 50mM NaCl, 10mM MgCl2, 150mM dTTP
amount, and so the weight/volume concentrations are used for these.) 6. Incubate at RT for 20min. Avoid prolonged incubations, as background can accumulate. 7. Wash coverslips 2-3 times with PBS, 3-5 min each wash, then rinse once with water. (I usually replace
Establishment of Stable Transfectant of CHO Lec Cells
expression vectors with strong promoter such as immediate early CMV (pcDNA3.1 etc.) or human EF-1a (pEF1/V5-His etc.). pBJ-5/GS vector also gives you high expression but requires additional cloning strategy. Materials Medium Sigma provides
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