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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Aluminum-nickel alloy
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
12635-27-7
- 规格:
A119429-500g/A119429-100g/A119429-25g
| 规格: | A119429-500g | 产品价格: | ¥543.9 |
|---|---|---|---|
| 规格: | A119429-100g | 产品价格: | ¥228.9 |
| 规格: | A119429-25g | 产品价格: | ¥72.9 |
银灰色无定型粉末(镍铝合金粉),具有中等程度的可燃性,有水存在的情况下部分活化并产生氢气易结块,长久暴露于空气中易风化。镍铝合金粉活化后为灰黑色颗粒,附有活泼氢,极不稳定,在空气中氧化燃烧,须浸在无水乙醇中保存。
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文献和实验6xHis-tagged protein purification using Qiagen Ni-NTA Column
)Centrifuge lysate at 10,000xg for 20’ at 40℃to pellet the cellular debris and save supernatant.Add 5μl 2xSB to 5μl Supernatant and store at 200℃for SDS-PAGE analysisBatch purification under Native ConditionsAdd 2.5 ml of the 50% Ni-NTA slurry to 10 ml cleared
6xHis-tagged protein purification using Qiagen Ni-NTA Column under Native Condit
)Centrifuge lysate at 10,000xg for 20’ at40℃to pellet the cellular debris and save supernatant.Add 5μl 2xSB to 5μl Supernatant and store at 200℃for SDS-PAGE analysisBatch purification under Native ConditionsAdd 2.5 ml of the 50% Ni-NTA slurry to 10 ml cleared
-DNA 50ug Yeast RNA in a 0.5mL tube 12) denature 95° 3 min 13) add to hybrid tube mix, hybridize at 65°C for 20-36 hrs Post hybridization: hybridize for 36-48 hrs wash 1) heat up 2X SSPE/0.1% SDS (wash) heat up to 65° C (?use
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