PE Anti-Mouse CD5 (53-7.3)

T-cell dependent antigen specific in situ staining (Pe)

6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block

Lung-Specific Expression of Mutant p53 as Mouse Model for Lung Cancer

The p53 tumor suppressor gene is the most commonly mutated gene in cancer (1 ,2) and is mutated in 50% non-small cell lung cancer (NSCLC) and 70% of small cell lung cancer (SCLC) (3) . Mutations in p53 commonly reflect exposures

Generation and Characterization of p53 Mutant Mice

. The emergence of technology facilitating the ablation of a gene within an animal’s genome allowed great advances in the study of p53 . The p53 knockout mouse was one of the first of its kind and provided a powerful tool for the study of p53. Production