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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
E. coli S30 Extract System for Linear Templates
- 供应商:
普洛麦格
- 规格:
30 reactions
| 说明 |
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E. coli S30 Extract System for Linear Templates ( 用于线性DNA 模板的E. coli S30 提取物系统) 的制备采用并稍加改进了Lesley 及其同事描述的操作方法,用于对线性DNA 模板进行转录/ 翻译反应。研究者只需提供含有一个原核E. coli 样的启动子( 如lac UV5, tac , λ PL (con) 和
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| 特点 | . 灵活:可使用多种模板:DNA 片段、PCR 合成的DNA、连接后的寡聚核苷酸、体外生成的RNA 和原核RNA。 |
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文献和实验Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels DNA Preparation E. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol., 174, 1992). Cells are embed allowfullscreen='true'ded in agarose
In vitro transcription with yeast nuclear extract
nuclear extract per reaction. The optimum amount depends on the extract used and the response is not always linear with amount of extract added). 5. After the 30-45 min incubation, add 180 microliters stop mix. 6. Extract 1x with phenol/chloroform
Invitro transcription with yeast nuclear extract
per reaction. The optimum amount depends on the extract used and the response is not always linear with amount of extract added). 5. After the 30-45 min incubation, add 180 microliters stop mix. 6. Extract 1x with phenol/chloroform
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