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- promega
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- L1030
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
30 reactions
| L1030 | E. coli S30 Extract System for Linear Templates | 30 reactions | 3,667 | |
| L1061 | pF25A ICE T7 Flexi® Vector | 20µg | 2,538 | |
| L1081 | pF25K ICE T7 Flexi® Vector | 20µg | 2,538 | |
| L1101 | TNT® T7 Insect Cell Extract Protein Expression System | 10 reactions | 2,044 | |
| L1102 | TNT® T7 Insect Cell Extract Protein Expression System | 40 reactions | 7,155 | |
| L1110 | S30 T7 High-Yield Protein Expression System | 24 reactions | 5,178 | |
| L1115 | S30 T7 High-Yield Protein Expression System | 8 reactions | 2,044 | |
| L1130 | E. coli T7 S30 Extract System for Circular DNA | 30 reactions | 3,868 | |
| L1170 | TNT® T7 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 |
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文献和实验Linear amplification of limiting amounts of RNA for gene expression studies
I, 1µl E. coli DNA Ligase, 2µl RNAse H; Add 130µl mix to the first strand cDNA (total volume 150µl), tap the tube gently to mix; Spin briefly and incubate at 16°C for 2 hours; Add 3µl of T4 DNA polymerase; Incubate at 16°C
In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani
or degradation of short-lived mRNA (5 , 6 ). The combined transcription/translation system from E. coli did not give satisfactory results with tetanus toxin (TeTx)-specific DNA (Fig. 1 ). Translation of in vitro transcribed TeTx-specific mRNA in reticulocyte
Introduction to the EMSA (Gel Shift) Technique
on linear DNA fragments result in the characteristic retarded mobility in the gel. However, if circular DNA is used (e.g., minicircles of 200-400 bp), the protein:DNA complex may actually migrate faster than the free DNA. Gel shift assays are also good
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