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- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
HDAC-Glo(TM) I/II Control Substrate
- 供应商：
普洛麦格
- 规格：
10µl
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文献和实验A general protocol for staining cell for cytometry analysis
Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture
A general protocol for staining cell for cytometry analysis
at RT. Staining 1.Wash cells twice with cold PBS and then resuspend cells in 1× Binding Buffer at a concentration of ~1 × 10 6 cells/ml. 2.Transfer 100 µl of the solution (~1 × 10 5 cells) to a 5 ml culture tube. 3. Add Annexin V and Vital Dye
;6.After the third wash the bound beads were eluted using elution buffer II, incubated at 65°C for 10 minutes and separated using the magnetic bead separator;7.The final ~100µl elute is then assessed for promoter specific DNA and/or RNA by PCR and RT-PCR respectively
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