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- 文献和实验
- 技术资料
- 供应商:
普洛麦格
- 英文名:
Asp-N, Sequencing Grade
- 规格:
2µg
Asp-N, Sequencing Grade(Asp-N,测序级 ) 是一种蛋白内切酶,能水解天冬氨酸残基 (Asp) 和半胱氨酸残基 (Cys) N 末端的肽键。当 pH 范围在 4.0-9.0 时, Asp-N 的活性最佳。该测序级蛋白酶可以单独使用,也可以与其它蛋白酶一起使用,以产生蛋白消化物,然后通过肽指纹图谱或 MS/MS 光谱匹配用于肽图谱或蛋白鉴定。该测序级蛋白酶适合于溶液或凝胶中的消化反应。
储存条件: 储存于 4℃。
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文献和实验ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5-methyl cytosine, and thymine, in a DNA oligonucleotide
initial primer is used that is N-1 in length. Repeating the initial cycling (steps 1-4) now generates an overlapping data set (bases 3/4, 8/9, etc, see Fig 5). Figure 5. Sequencing coverage during SOLiD sequencing cycles. Again this is original
N-Terminal Amino Acid Sequencing of 2-DE Spots
Two-dimensional electrophoresis (2-DE) is one of the most advanced techniques for direct separation of considerable numbers of proteins. Direct microsequence analysis of the amino (N)-terminal partial sequence of the electroblotted protein
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