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- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
MB 488 NHS ester-100 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
MB 488 NHS ester-100mg,MB 488 NHS ester-100 mg,MB 488 NHS ester-100mg,MB 488 NHS ester-100 mg
MB 488 NHS ester-100mg-MB 488 NHS ester-100 mg
产品货号:CCT-FP-1601-100
产品规格:100mg
MB 488 NHS酯是一种非常明亮的亲水性绿色荧光染料,用于通过伯胺(Lys)标记蛋白质和抗体。

MB™ 488 is a very hydrophilic, water-soluble green emitting dye. Structurally related to Alexa Fluor® 488 dye, MB™ 488 dye is a next step in improving water solubility, brightness and minimizing self-quenching. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™ 488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications. The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

MB 488 NHS ester-100mg-MB 488 NHS ester-100 mg-20°C.
点击:MB 488 NHS ester-100mg-MB 488 NHS ester-100 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
. Properties of Fluorescent Dyes Used for Tubulin Labeling Back to protocols I. Solutions & Supplies phosphocellulose-purified tubulin (~50 mg = 2-4 3 ml aliquots of 5-10 mg/ml PC fractions) Dye stock in anhydrous DMSO (20-100 mM
完全培养基轻轻吹打10-15次重悬。 6.【传代】按1:3-1:4接种至新瓶,补加完全培养基至工作体积,十字混匀,标注名称、代次、日期。 7.【镜检】镜下确认分散均匀,放入37℃、5%CO2培养箱,24-48h观察细胞状态。 注意:MDA-MB-231增殖快,通常约48h可达汇合,切勿过度融合;胰酶消化1-3min即可,过度消化降低存活,吹打需轻柔。 三、细胞冻存 1.【准备工作】冻存液:无血清细胞冻存液(abs9417-100mL)或90% FBS(货号:abs972-500mL/SV30302
Coupling Peptides To Resin For Affinity Purification
for 10 min at RT. The resin has about 10 5mol groups/ml, so add about 20 5mol reagent per ml (7 mg/ml resin). Dissolve the IAA-NHS ester in dry DMSO and add it while stirring the resin. This step and subsequent steps up to the blocking of residual
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