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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
AZDye 488 TFP ester-3 x 100 μg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 488 TFP酯-3 x 100 μ g,AZDye 488 TFP ester-3 x 100 μg,AZDye 488 TFP酯-3 x 100 μ g,AZDye 488 TFP ester-3 x 100 μg
AZDye 488 TFP酯-3 x 100 μ g-AZDye 488 TFP ester-3 x 100 μg
产品货号:CCT-FP-1026-100
产品规格:3x100ug
AZDye™ 488 TFP酯是一种胺反应性发绿光染料,通常用于通过伯胺(Lys)、胺修饰的寡核苷酸和其他含胺生物分子标记蛋白质或抗体。标记在pH 7-9时最有效,并形成稳定的共价酰胺键。AZDye™ 488缀合物是具有非常高的荧光量子产率的高度光稳定的染料。AZDye™ 488在结构上与Alexa Fluor® 488相同。它的吸收/发射光谱与许多其他基于磺化罗丹明110核心的荧光染料的光谱完美匹配,包括DyLight® 488,Alexa Fluor® 488。

AZDye™ 488 TFP Ester is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488 Dye.Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AZDye 488 TFP酯-3 x 100 μ g-AZDye 488 TFP ester-3 x 100 μg-20°C.
点击:AZDye 488 TFP酯-3 x 100 μ g-AZDye 488 TFP ester-3 x 100 μg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验珠子对照样品外每个样品中加入一抗,抗体的量按照以往经验来加,1-10ug的抗体/25ugDNA效果较好。3加入20ul的proteinA/G珠子(预先用超声处理成为单链的鲱精DNA和BSA吸附,详见步骤4.3a)到所有样品总,4°C摇转IP过夜。3a将蛋白A/G珠子与单链鲱精DNA进行预处理。如果同时使用蛋白A珠子和蛋白G珠子,等体积混合这两种珠子使用RIPA溶液洗3次。除去RIPA溶液,加入单链鲱精DNA到珠子终浓度为75ng/μl,再用BSA将珠子终浓度定到0.1μg/μl。加入两倍珠子
试剂 考马斯亮蓝G-250 的配制:称取100mg 考马斯亮蓝G-250,溶于50mL90%乙醇中,加入85%(W/V)的磷酸100mL,最后用蒸馏水定容到1 000mL。此溶液在常温下可放置一个月。 (3)乙醇 (4)磷酸(85%)四、操作步骤 1.标准曲线制作 (1)0~100μg/mL 标准曲线的制作:取6 支10mL 干净的具塞试管,按表1 取样。盖塞后,将各试管中溶液纵向倒转混合,放置2min 后用1cm
冰上,置水平摇床上) 4. 4℃,14000g离心15min,立即将上清转移到一个新的离心管中 5. 准备Protein A agarose,用PBS 洗两遍珠子,然后用PBS配制成50%浓度,建议减掉枪尖部分,避免在涉及琼脂糖珠的操作中破坏琼脂糖珠 6. 每1ml总蛋白中加入100μl Protein A琼脂糖珠(50%),4℃摇晃10min(EP管插冰上,置水平摇床上),以去除非特异性杂蛋白,降低背景 7. 4℃,14000g离心15min,将上清转移到一个新的离心管中,去除Protein
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