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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
DBCO-PEG5-NHS Ester-4 x 2 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
DBCO-PEG 5-NHS酯-4 x 2 mg,DBCO-PEG5-NHS Ester-4 x 2 mg,DBCO-PEG 5-NHS酯-4 x 2 mg,DBCO-PEG5-NHS Ester-4 x 2 mg
DBCO-PEG 5-NHS酯-4 x 2 mg-DBCO-PEG5-NHS Ester-4 x 2 mg
产品货号:CCT-A102P-2
产品规格:4x2mg
DBCO-PEG 5-NHS酯与伯胺(例如,赖氨酸残基的侧链或氨基硅烷包被的表面)在pH 7-9下形成共价键。亲水性聚乙二醇(PEG)间隔臂赋予水溶性,其被转移到标记分子,从而减少储存在溶液中的标记蛋白质的聚集。PEG间隔臂提供长且柔性的连接,其最小化与互补的含叠氮化物分子的连接有关的空间位阻。

DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

DBCO-PEG 5-NHS酯-4 x 2 mg-DBCO-PEG5-NHS Ester-4 x 2 mg-20°C. Desiccate
点击:DBCO-PEG 5-NHS酯-4 x 2 mg-DBCO-PEG5-NHS Ester-4 x 2 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
Preparation of Segmented and Polarity Marked Microtubules
well for us using tetramethylrhodamine NHS ester-labeled tubulin (stoichiometry of labeling ~1.4). I. Solutions & Supplies II. Segmented Taxol Microtubules III. Polarity Marked Taxol Microtubules
Preparation of Segmented and Polarity Marked Microtubules
on the labeled tubulin prep. Described here is what has worked well for us using tetramethylrhodamine NHS ester-labeled tubulin (stoichiometry of labeling ~1.4). I. Solutions & Supplies II. Segmented Taxol Microtubules
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