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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
CalFluor 555 Azide-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
CalFluor 555Azide-1 mg,CalFluor 555 Azide-1 mg,CalFluor 555Azide-1 mg,CalFluor 555 Azide-1 mg
CalFluor 555Azide-1 mg-CalFluor 555 Azide-1 mg
产品货号:CCT-1370-1
产品规格:1mg
CalFluor 555 Azide是一种荧光叠氮化物探针,可通过Cu催化或无金属点击反应激活。这种叠氮化物探针在与炔反应之前不发荧光。CalFluor 555叠氮化物是水溶性的,其激发带与532 nm或555 nm激光线很好地匹配。可与标准TRITC(四甲基罗丹明)过滤器组一起使用。

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

CalFluor 555Azide-1 mg-CalFluor 555 Azide-1 mg-20°C. Desiccate
点击:CalFluor 555Azide-1 mg-CalFluor 555 Azide-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
. 7.TRITC,10% on celite (Research Organics). 8.Bio-Beads SM-2 in 0.7x15 column. Procedure (perform under reduced light) 1.Get 1-2 mg vinculin,stored in 2 mM Pipes,0.02% Azide,pH 7.6,in liquid nitrogen.Thaw quickly in warm water and chill in ice. 2.Mix
LABELING ALPHA-ACTININ WITH IATR
. 8.IATR (tetramethylrhodamine iodoacetamide; Moledular Probes). Procedure (perform under reduced light) 1.Get 2 mg gizzard alpha-actinin,stored in 2 mM PIPES,0.02% azide,pH 7.0 in liquid nitrogen.Thaw quickly in warm water and chill in ice. 2.Resuspend
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