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- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 568 Alkyne-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 568 Alkyne-1mg,AZDye 568 Alkyne-1 mg,AZDye 568 Alkyne-1mg,AZDye 568 Alkyne-1 mg
AZDye 568 Alkyne-1mg-AZDye 568 Alkyne-1 mg
产品货号:CCT-1293-1
产品规格:1mg
AZDye™ 568 Alkyne是一种水溶性、明亮的红色荧光染料,其激发非常适合568 nm激光线。AZDye™ 568炔通过铜催化的点击反应(CuAAC)与叠氮化物反应以形成稳定的三唑连接基。用于无铜叠氮化物检测的探针(AZDye™ 568 DBCO)也可用于不允许存在铜的应用。

AZDye™ 568 Alkyne is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 568 Dye and Alexa Fluor® 568.For application where the presence of copper is not acceptable, please consider our AZDye™ 568 DBCO probes for copper-less detection of azide-modified molecules.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AZDye 568 Alkyne-1mg-AZDye 568 Alkyne-1 mg-20°C. Desiccate
点击:AZDye 568 Alkyne-1mg-AZDye 568 Alkyne-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验液裂解细胞,用移液器适当吹打使细胞脱落,大瓶贴壁细胞可以先用胰酶洗脱下来后再裂解,裂解方法仿照悬浮细胞处理。 B、 悬浮细胞的处理:先将悬浮细胞置于 EP 管中 3000 rpm 离心 5 min 去掉上清培养基,每 20 μL 体系细胞加 100 μL 裂解液反复吹打至细胞完全破碎。 处理好的样品 12000 rpm 离心 5 min,取上清即为提取的蛋白。置于 4 ℃ 可保存一周,-20℃ 可保存约 2 个月,-80 ℃ 可保存半年。 蛋白浓度的测定:酶标板,酶标仪、离心机、1 mg/mL
Ribonuclease A (RNase A), DNase-free
The absence of endodeoxyribonucleases, exodeoxyribonucleases and proteases confirmed by appropriate quality tests. Functionally tested for RNA digestion in a plasmid DNA purification procedure.Concentration10 mg/ml.Protein concentration is determined
Procedure for fixing and staining adherent cells for immunofluorescence
serum is for blocking purposes and should correspond to species that secondary antibody is developed in. For example, if you are using goat anti-rabbit 488 and goat anti-mouse 568, you should use 5 normal goat serum for blocking 8. Place 100uL
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