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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 488 Azide-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 488 Azide-1 mg,AZDye 488 Azide-1 mg,AZDye 488 Azide-1 mg,AZDye 488 Azide-1 mg
AZDye 488 Azide-1 mg-AZDye 488 Azide-1 mg
产品货号:CCT-1275-1
产品规格:1mg
"AZDye ™ 488叠氮化物是一种明亮的绿色荧光叠氮化物激活探针,通过铜催化点击反应(CuAAC)与末端炔反应。 它还通过无铜点击化学反应与应变环辛炔反应以形成稳定的三唑,并且不需要Cu催化剂或升高的温度。 AZDye ™ 488是一种明亮、高光稳定性的绿色荧光探针,可由488 nm激光线最佳激发。 该探针是水溶性的,并且其荧光在宽pH范围内不依赖于pH。 蓝色染料的亮度和光稳定性最适合于低丰度目标的直接成像。 AZDye ™ 488在结构上与Alexa Fluor ® 488叠氮化物相同。 它的吸收/发射光谱与许多其他基于磺化罗丹明110核心的荧光染料的光谱完美匹配,包括DyLight ® 488,Alexa Fluor ® 488和CF ® 488 Dye。

AZDye™ 488 Azide, an equivalent of Alexa Fluor 488® Azide, is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A. AZDye™ 488 Alkyne can be used a less expensive alternative to Alexa Fluor® 488 Alkyne.Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AZDye 488 Azide-1 mg-AZDye 488 Azide-1 mg-20°C. Desiccate
点击:AZDye 488 Azide-1 mg-AZDye 488 Azide-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
PBS/azide. Resuspend the cells in propidium iodide at 50 µg/ml in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for FITC, PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear
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