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- 美国
- CCT-QBD-10325-1000
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
2年
- 英文名：
Bis-dPEG®₃-biotin-1000 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
Bis-dPEG® β-生物素-1000 mg，Bis-dPEG®₃-biotin-1000 mg，Bis-dPEG® β-生物素-1000 mg,Bis-dPEG®₃-biotin-1000 mg
Bis-dPEG® β-生物素-1000 mg-Bis-dPEG®₃-biotin-1000 mg
产品货号：CCT-QBD-10325-1000
产品规格：1000mg
Bis-dPEG®3-biotin(产品编号QBD-10325)是一种新型生物素标记产品，由dPEG®3间隔区和连接在间隔区两端的生物素组成。Bis-dPEG®3-biotin可与两个不同的抗生物素蛋白/链霉亲和素结合位点(分子内和分子间)结合，为客户提供了利用生物素-抗生物素蛋白/链霉亲和素亲和素亲和力的独特机会。事实上，该产品专门用于阻断所有细胞和组织中发现的内源性生物素，因此在已知内源性生物素会引起问题的治疗和诊断应用中，它不会干扰靶向生物素-链霉亲和素相互作用。
Bis-dPEG®3-biotin, product number QBD-10325, is a novel biotin-labeling product composed of a dPEG®3 spacer with biotin attached to each end of the spacer, Bis-dPEG®3-biotin offers customers unique opportunities for exploiting the biotin-avidin/streptavidin affinity since it can bind to two different avidin/streptavidin binding sites, both intramolecularly and intermolecularly. Indeed, this product was developed specifically to block endogenous biotin found in all cells and tissues so that it did not interfere with targeted biotin-streptavidin interactions in therapeutic and diagnostic applications where endogenous biotin was known to cause problems.The binding behavior of Bis-dPEG®3-biotin with streptavidin opens up possible uses for the product in the self-assembly of supramolecular constructs and signal amplification. In the first example, if two parts of a potential supramolecular construct both contain avidin or streptavidin, addition of QBD-10325 under empirically determined optimized conditions could trigger self-assembly of the construct as the bis-dPEG®3-biotin binds to the separate avidin or streptavidin molecules in the two halves of the construct. In the second example, multiple streptavidin molecules conjugated to horseradish peroxidase (HRP) could be bound together with a streptavidin-biotinylated protein complex (for example, a biotinylated antibody), thus amplifying the signal from HRP.
Bis-dPEG® β-生物素-1000 mg-Bis-dPEG®₃-biotin-1000 mg
点击：Bis-dPEG® β-生物素-1000 mg-Bis-dPEG®₃-biotin-1000 mg查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。
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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
-Tactin Superflow® columns with a column volume (CV) of 200 µl (IBA Tagnology, Cat. No. 2-1207-550) (see note 6 ). Purification is performed at 4°C in a cold room. Equilibrate the column by applying 2x CV of Washing buffer . Add 5-10 mg
Purification of Human Multiprotein Complexes using OneSTrEP Technology
One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone chaperone Asf1 complexes (Groth et al., 2007), and we have recently optimized it further. Using this protocol we routinely obtain complexes in amounts
一、原理1、E .coli 表达系统E .coli 是重要的原核表达体系。在重组基因转化入E .coli 菌株以后，通过温度的控制，诱导其在宿主菌内表达目的蛋白质，将表达样品进行SDS-PAGE 以检测表达蛋白质。2、外源基因的诱导表达提高外源基因表达水平的基本手段之一，就是将宿主菌的生长与外源基因的表达分成两个阶段，以减轻宿主菌的负荷。常用的有温度诱导和药物诱导。本实验采用异丙基硫代-β-D-半乳糖昔（IPTG）诱导外源基因表达。不同的表达质粒表达方法并不完全相同，因启动子不同，诱导
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