- ￥4692
- BPS Bioscience
- 美国
- 52084
- 2025年11月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Protein
- 供应商：
盛祺兆生物
- 规格：
50 µg
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文献和实验(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
should also be included. In Ringrose et al ., (2004) I used histone H3 peptides spanning residues 1-13 (for lysine 4 and lysine 9 modifications) or 21-33 (for lysine 27 modifications). Stock solutions of 0.5mM peptide in PBS can be prepared and kept at -20°C
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
should be conjugated to the N-termini of C-terminal histone peptides and the C-termini of N-terminal histone peptides For a negative control: corresponding unmodified peptides should be synthesized for each modified residue For a positive control: known
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