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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 英文名:
3-(4-(tert-Butyl)pyridinio)-1-propanesulfonate
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
570412-84-9
- 规格:
10 mM * 1 mL/1 g
| 规格: | 10 mM * 1 mL | 产品价格: | ¥289.0 |
|---|---|---|---|
| 规格: | 1 g | 产品价格: | ¥263.0 |
产品活性:NDSB 256-4T 是一种非洗涤性磺胺甜菜碱化合物。NDSB-256-4T 通过与早期折叠中间体相互作用阻止蛋白质聚集并促进蛋白质折叠。
产品来源: www.medchemexpress.cn/ndsb-256-4t.html
研究领域:Others
作用靶点:Biochemical Assay Reagents
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文献和实验Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells
at the tight junction in experimental models. One of its key effectors is the transcription factor, �IRF-1. In our studies of the role of IRF-1 in barrier function using the human T84 intestinal epithelial cell monolayer model, we have found that induction
Preparation of active proteins from inclusion bodies.
NDSB256 (or 1M NDSB201). the final protein concentration should not exceed 0.05 to 0.1 mg/ml. A fast an efficient mix is essential. for small volumes dispensing the protein solution with a pipette directly into the folding buffer while vortexing
允许,克隆到T载体上再酶切。很多PCR产物酶切连接不上的问题克隆到载体上后再酶切都没有问题了。 2、片段测过序吗?内部是否有这两个酶? wangch84 tozitong1983,感谢您的回答,很专业。 1. bamh1和ecor1这两个酶是我个人比较偏爱的酶。我构建过十多个重组质粒,几乎都是用这两种酶,一直也没出现过问题(pet30a载体上这两个酶是紧挨着的,重组质粒构建的也很顺利。)我查了载体谱图,hind3我用不了,而且bamh1和ecor
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