鸡输卵管上皮细胞

二、细胞培养状态
发货时发送细胞电子版照片
在技术部标准操作流程下，建议您收到细胞后尽快进行相关实验。
使用方法
1. 取出T25细胞培养瓶，用75%酒精消毒瓶身，拆下封口膜，放入37℃、5%CO2、饱和湿度的细胞培养箱中静置3-4h，以稳定细胞状态。
2. 贴壁细胞消化
1)吸出T25细胞培养瓶中的培养基，用PBS清洗细胞一次；
2)添加0.25%胰蛋白酶消化液1mL至T25培养瓶中，轻微转动培养瓶至消化液覆盖整个培养瓶底后，吸出多余胰蛋白酶消化液，37℃温浴1-3min；倒置显微镜下观察，待细胞回缩变圆后，再加入5ml完全培养基终止消化；
3)用吸管轻轻吹打混匀，按传代比例接种T25培养瓶传代，然后补充新鲜的完全培养基至5mL，置于37℃、5%CO2、饱和湿度的细胞培养箱中静置培养；
4)待细胞完全贴壁后，培养观察；之后按照换液频率更换新鲜的完全培养基。          

3. 细胞收货脱落
1)收集所有细胞悬液，1000rpm，离心5min，保留沉淀；
2)添加0.25%胰蛋白酶消化液0.5mL至离心管中，重悬沉淀，放置于37℃消化3min(或4℃冰箱静置5-7min)；消化完向离心管内加入5ml完全培养基终止消化；
3)经1000rpm，离心5min，丢弃上清，用5ml完全培养基重悬沉淀，接种于新的培养瓶内；
4)待细胞完全贴壁后，培养观察；之后按照换液频率更换新鲜的完全培养基(37℃预热)。
5)原瓶内贴壁细胞按正常消化处理。
4. 细胞实验
因原代细胞贴壁特殊性，贴壁的原代细胞在消化后转移至其他实验器皿（如玻璃爬片、培养板、共聚焦培养皿等）时，需要对实验器皿进行包被，以增强细胞贴壁性，避免细胞因没贴好影响实验；包被条件常选用鼠尾胶原Ⅰ（2-5μg/cm2） ，多聚赖氨酸PLL（0.1mg/ml），明胶（0.1%），依据细胞种类而定。悬浮/半悬浮细胞无需包被。
公司正在 销售的产品：
Anti-OGFOD1 Antibody (Clone#OTI1F4)　Recombinant Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)
Anti-RAD51L1/RAD51B Antibody (Clone#OTI7H4)　Recombinant SRSF Protein Kinase 2 (SRPK2)
Anti-ERCC2 Antibody (Clone#OTI4B11)　Recombinant Aminopeptidase A (ENPEP)
Anti-CD5 Antibody　Recombinant Carboxypeptidase N2 (CPN2)
Anti-CDX2 Antibody　Recombinant Adenylate Cyclase 8, Brain (ADCY8)
Anti-RNASE11 Antibody (Clone#OTI2C11)　Recombinant Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS)
Anti-Golgin 97/GOLGA1 Antibody　Recombinant Deoxyribonuclease II (DNASEII)
Anti-KIF9 Antibody (Clone#OTI1A2)　Recombinant Tyrosyl tRNA Synthetase (YARS)
Anti-CD73/NT5E Antibody　Recombinant Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1)
Anti-XRCC1 Antibody (Clone#1E11)　Recombinant Cysteine Dioxygenase I (CDO1)
Anti-DFF45/DFFA Antibody (Clone#OTI5C4)　Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2)
Anti-GLUT3/SLC2A3 Antibody　Recombinant Related To Receptor Tyrosine Kinase (RYK)
Anti-CD11B/Integrin Alpha M/ITGAM Antibody　Recombinant BMX Non Receptor Tyrosine Kinase (BMX)
Anti-SULT2A1 Antibody (Clone#OTI3G4)　Recombinant BMX Non Receptor Tyrosine Kinase (BMX)
Anti-CD79B Antibody (Clone#27C14)　Recombinant Ribonuclease A9 (RNASE9)
Anti-EIF3D Antibody (Clone#OTI1G10)　Recombinant Peptidase Inhibitor 16 (PI16)
Anti-SUMF1 AntibodAnti-OGFOD1 Antibody (Clone#OTI1F4)　Recombinant Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)
Anti-RAD51L1/RAD51B Antibody (Clone#OTI7H4)　Recombinant SRSF Protein Kinase 2 (SRPK2)
Anti-ERCC2 Antibody (Clone#OTI4B11)　Recombinant Aminopeptidase A (ENPEP)
Anti-CD5 Antibody　Recombinant Carboxypeptidase N2 (CPN2)
Anti-CDX2 Antibody　Recombinant Adenylate Cyclase 8, Brain (ADCY8)
Anti-RNASE11 Antibody (Clone#OTI2C11)　Recombinant Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS)
Anti-Golgin 97/GOLGA1 Antibody　Recombinant Deoxyribonuclease II (DNASEII)
Anti-KIF9 Antibody (Clone#OTI1A2)　Recombinant Tyrosyl tRNA Synthetase (YARS)
Anti-CD73/NT5E Antibody　Recombinant Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1)
Anti-XRCC1 Antibody (Clone#1E11)　Recombinant Cysteine Dioxygenase I (CDO1)
Anti-DFF45/DFFA Antibody (Clone#OTI5C4)　Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2)
Anti-GLUT3/SLC2A3 Antibody　Recombinant Related To Receptor Tyrosine Kinase (RYK)
Anti-CD11B/Integrin Alpha M/ITGAM Antibody　Recombinant BMX Non Receptor Tyrosine Kinase (BMX)
Anti-SULT2A1 Antibody (Clone#OTI3G4)　Recombinant BMX Non Receptor Tyrosine Kinase (BMX)
Anti-CD79B Antibody (Clone#27C14)　Recombinant Ribonuclease A9 (RNASE9)
Anti-EIF3D Antibody (Clone#OTI1G10)　Recombinant Peptidase Inhibitor 16 (PI16)
Anti-SUMF1 Antibody (Clone#OTI1B10)　Recombinant Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG)
Anti-ACVR1B Antib鸡输卵管上皮细胞ehydrogenase A (LDHA)
Anti-MCM2 Antibody (Clone#11C4)　Recombinant Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG)
Anti-FUK/FCSK Antibody (Clone#6E2)　Recombinant Aminopeptidase A (ENPEP)
Anti-LIMS1 Antibody (Clone#5G7)　Recombinant Adenylate Cyclase 9 (ADCY9)
Anti-KIR3.1/KCNJ3 Antibody (Clone#OTI1C5)　Recombinant Ubiquitin Specific Peptidase 7 (USP7)
ombinant Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG)
Anti-ACVR1B Antibody (Clone#23A40)　Recombinant Lactate Dehydrogenase A (LDHA)
Anti-MCM2 Antibody (Clone#11C4)　Recombinant Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG)
Anti-FUK/FCSK Antibody (Clone#6E2)　Recombinant Aminopeptidase A (ENPEP)
Anti-LIMS1 Antibody (Clone#5G7)　Recombinant Adenylate Cyclase 9 (ADCY9)
Anti-KIR3.1/KCNJ3 Antibody (Clone#OTI1C5)　Recombinant Ubiquitin Specific Peptidase 7 (USP7)
四、注意事项
1. 培养基于4℃条件下可保存3-6个月。
2. 在细胞培养过程中，请注意保持无菌操作。
3. 传代培养过程中，胰酶消化时间不宜过长，否则会影响细胞贴壁及其生长状态。
4. 建议客户收到细胞后前3天每个倍数各拍几张细胞照片，记录细胞状态，便于和技术部沟通；由于运输的原因，个别敏感细胞会出现不稳定的情况，请及时和我们联系，详尽告知细胞的具体情况，以便我们的技术人员跟踪、回访直至问题得到解决。   ​​​​​​​