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- Sigma
- 进口
- SML2357-5MG
- 2025年09月28日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
见标签
- 库存：
现货
- 供应商：
鹿森
- CAS号：
241479-67-4
- 规格：
5mg
应用
to analyze its in vitro activity against isolates from the R. argillacea species complex A. fumigatus, N. hiratsukae and C. parapsilosis.
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文献和实验0,02% sodium thiosulfate for 1 min.5)Rinse with water for 1 min.2X.0.02% Sodium Thiosulfate: For 1 gel,add 30mg sodium thiosulfate-5 hydrate to 150ml water.Silver reaction6)Submerge gel in 150ml 0.1% silver nitrate with 0.08% formalin (37%)for 20 min
T-cell dependent antigen specific in situ staining (Pe)
) Levamisole 1mM (12 mg/50 ml) (Sigma L9756) 20 mg Naphtol As BI phosphate (stock solution 40 mg/ml in NN-DM formamide, anhydrous, kept at -20°C) (Sigma N2250, the cheapo) 10 mg Fast Blue BB Diazonium salt (Sigma F3378) Shake
in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min
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