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文献和实验Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
Harvest Immune Complexes 1. To each tube, add: 4 µl sonicated lambda DNA (Gibco BRL cat#25250-010, must be sonicated like the ChIP extracts & check on gel to verify small size-add azide to 0.01%) 40 µl Protein A Sepharose Beads (prepared as 50% slurry
来电来函咨询，我们将以最大的热忱为您服务！参考文献：1. Q. Wang, T. R. Chan, R. Hilgraf, V. V. Fokin, K. B. Sharpless, M. G. Finn. Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition. J. Am. Chem. Soc., 2003, 125 (11): 3192-3193.2. C. W. Tornoe, C. Christensen
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