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文献和实验were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
., Alexa Fluor 488 carboxylic acid, succinimidyl ester [Invitrogen]; FITC [Thermo Fisher Scientific]; rhodamine‐maleimide [Biotium]) DMSO, anhydrous grade
Overview of Biacore Systems and Their Applications
, S., Wyatt, G., Quinn, J., and O'Kennedy, R. 2005. Development of a surface plasmon resonance‐based immunoassay for Listeria monocytogenes. J. Food Prot. 68:728‐735. Lopez
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