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文献和实验alkyne?azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini?DNA duplexes. The reaction is carried out on 5??alkyne?3??azide?labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
,000 pfus of lambda-gt11 (diluted in TMG) in glass test tubes and incubate at 37 - 42°C for 15 minutes without shaking. Plate this mixture with 7 - 7.5 ml top agar per 150 mm Petri dish. Let the dishes sit on the lab bench for about 5 minutes
Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
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