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上海玉博生物科技有限公司
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文献和实验Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation&
with two or three fluorochrome-conjugated antibodies. RNase should be added for DNA dyes that can bind dsRNA (such as propidium iodide). 70% EtOH in ddH2 0 50% fetal bovine serum (FBS) in PBS with 0.1% sodium azide 70% EtOH in glycerol staining buffer (2% FBS in PBS
Simultaneous analysis of DNA content and surface
Volume 46: Cell Death , B. Osborne and L. Schwartz, eds., Academic Press, New York, NY, pp. 57-76. Prepare the cell type of interest as a single cell suspension and wash once with cold PBS with 0.1% sodium azide. Label
Amicon Stirred Ultrafiltration Cells
400 10.0 76 41.8 1.5 Maximum operating pressure: 75 psi (5.3 kg/cm²) Relief valve setting: 90 psi (6.3 kg/cm²) Maximum diafiltration operating pressure: 55 psi (3.9 kg/cm²) ¹ Minimum
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