Broadpharm代理商-diSulfo-Cy7-bis

Coupling Peptides To Resin For Affinity Purification

5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate

Coupling Peptides To Resin For Affinity Purification

volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add

Revised amino-allyl labeling protocol

, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution.     3. Add 450 m L water to each reaction.     C. Cleanup     Add 500 m L neutralized, diluted reaction