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文献和实验Sequence-specific protein/DNA contacts direct most transcription factors to binding sites within the promoters of genes they regulate. Several chemical probes, such as dimethyl sulfate, have been used to obtain information on these sites
Dimethyl sulfate (DMS) is an effective and widely used probe for sequence-specific protein-DNA interactions. It is the only probe routinely used both for in vitro (methylation protection, methylation interference) and in vivo (DMS genomic
Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, and wean heroin addicts. Measurement of methadone and its major metabolite EDDP in urine is useful for assessing compliance with addiction rehabilitation and pain
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