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上海玉博生物科技有限公司
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文献和实验TRICINE GELS Protocol (For Low MW proteins (
% Acrylamide solution 19:1 (Acrylamide 19 / Methylene bis Acrylamide 1) for short peptides. Keep 4°C. 3) Glycerol 70% 4) Cathode buffer 10X (upper buffer): 1M Tris + 1M Tricine + 1% SDS pH 8.25. Keep RT. (Prepare: 24.2gr Tris + 35.84gr Tricine + 2gr
TRICINE GELS Protocol (For Low MW proteins (
% Acrylamide solution 19:1 (Acrylamide 19 / Methylene bis Acrylamide 1) for short peptides. Keep 4°C. 3) Glycerol 70% 4) Cathode buffer 10X (upper buffer): 1M Tris + 1M Tricine + 1% SDS pH 8.25. Keep RT. (Prepare: 24.2gr Tris + 35.84gr Tricine + 2gr
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
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