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文献和实验TRICINE GELS Protocol (For Low MW proteins (
Solution Methanol CP 500ml 50% Acetic Acid CP 100ml 10% H2O 400ml
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
How to Make Simple Solutions and Dilutions
than the desired dilution factor value. Example 2: Suppose you must prepare 400 ml of a disinfectant that requires 1:8 dilution from a concentrated stock solution with water. Divide the volume needed by the dilution factor (400 ml / 8 = 50 ml) to determine
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