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文献和实验the cell pellets in a total of 70 ml of GET/Lysozyme solution (35 ml for each bottle) by gently teasing the pellet with a spatula and incubate for 10 minutes at room temperature. (Note: Do not vortex the lysate at any time because this may shear
Streptomyces:Protocols/Mini-Maxi Prep
, forming a biphasic mixture. The proteins partition into the organic phase while the DNA (as well as other contaminants such as salts, sugars, etc.) remain in the aqueous phase. This (top) phase is removed and the DNA precipitated with alcohol; all anionic
100 μl 1 M MgCl2 330 μl ddH2 O 1 ml 2.7 M MOPS (acid form): 5.65 g MOPS (acid form) ddH2 O to 10 ml MOPS-Acid buffer: 1.35 M MOPS (acid form), 100 mM MgCl2 in double distilled water. 500 μl 2.7 M MOPS (acid form) 100 μl 1 M MgCl2 400 μl
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