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- bs-0296G-BF594
- 2026年10月10日
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100ul/1ml
| 规格： | 100ul | 产品价格： | ￥420.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0296G-BF594 |
| 英文名称 | Goat Anti-Mouse IgG H&L, BF594 conjugated |
| 中文名称 | BF594标记的山羊抗小鼠IgG H&L |
| 英文别名 | Goat Anti-Mouse IgG H&L (BF594); Immunoglobulin G; |
| 产品应用 | IF=1:200-1000, Flow-Cyt=1:50-200, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Mouse |
| 抗体来源 | Goat |
| 免疫原 | Native Mouse IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:200-1000} |
| {Flow-Cyt} | {1:50-200} |
| {ICC/IF} | {1:100-1000} |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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