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GATA1 Recombinant Rabbit mAb(b

sm-54013R)-50ul/100ul/25ul
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  • ¥800 - 2500
  • Bioss已认证
  • bsm-54013R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/25ul

    规格:50ul产品价格:¥1400.0
    规格:100ul产品价格:¥2500.0
    规格:25ul产品价格:¥800.0
    产品编号bsm-54013R
    英文名称GATA1 Recombinant Rabbit mAb
    中文名称珠蛋白转录因子1抗体
    英文别名GATA1; ERYF 1; ERYF1 antibody Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1; GF 1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NFE 1; NFE1; GATA1_HUMAN; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein.
    产品应用WB=1:500-2000, Flow-Cyt=1:50-100, ICC/IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原Recombinant protein within human GATA1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量45 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Developmental Biology > Organogenesis > Hematopoietic system development

    Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger

    Stem Cells > Hematopoietic Progenitors > Intracellular Molecules

    亚基May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1.
    亚细胞定位Nucleus.
    组织特异性Erythrocytes.
    翻译后修饰Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    相似性Contains 2 GATA-type zinc fingers.
    功能Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {Flow-Cyt}{1:50-100}
    {ICC/IF}{1:50-200}

     

    产品细节图片1
    4% P‌‌araformaldehyde-fixed Hel.92.1.7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GATA1) monoclonal Antibody, unconjugated (bsm-54013R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
    产品细节图片2
    25 ug total protein per lane of various lysates (see on figure) probed with GATA1 monoclonal antibody, unconjugated (bsm-54013R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 2.5 }}] {Jie Luo. et al. Xin Sheng Hua Granule ameliorate chemotherapy-induced blood deficiency syndrome through ihibiting JAK1/STAT1 pathway activation. FITOTERAPIA. 2025 Jun;183:106571} {IHC,WB} {Mouse}

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    文献支持
    GATA1 Recombinant Rabbit mAb(bsm-54013R)-50ul/100ul/25ul
    ¥800 - 2500