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Heme Oxygenase 1 Recombinant R

abbit mAb(bsm-60751R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-60751R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-60751R
    英文名称Heme Oxygenase 1 Recombinant Rabbit mAb
    中文名称血红素氧合酶 1抗体
    英文别名Heme oxygenase(decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN.
    产品应用WB=1:2000-20000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, spleen, spleen, spleen, spleen, thymus, thymus, thymus, thymus, human lung carcinoma, human lung carcinoma, human lung carcinoma, human lung carcinoma, 肝, 肝, 肝, 肝, spleen, spleen, spleen, thymus, thymus, thymus
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Heme Oxygenase 1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量32 kDa
    浓度1mg/ml
    储存液PBS, Glycerol, BSA.
    研究领域

    Cancer > Cancer Metabolism > Response to hypoxia

    Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators

    Cardiovascular > Blood > Platelets

    Cardiovascular > Vasculature > Endothelium

    Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > NFkB pathway

    Metabolism > Pathways and Processes > Metabolism processes > Hypoxia

    Metabolism > Types of disease > Cancer

    Metabolism > Types of disease > Heart disease

    Metabolism > Types of disease > Neurodegenerative disease

    Neuroscience > Neurology process > Neurodegenerative disease > Alzheimer's disease

    Signal Transduction > Signaling Pathway > Nuclear Signaling > NFkB Pathway

    亚细胞定位Microsome. Endoplasmic reticulum.
    组织特异性Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
    相似性Belongs to the heme oxygenase family.
    功能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The hemeoxygenase-1 calls that the hemoglobin oxidizes to synthesize the enzyme again-1( hemeoxygenase-1, HO-1) is the catalyst enzyme that a kind of hemoglobin declines the solution, under the NADPH and the cell dye P-450 revivification enzymes and the member oxygen functions, the catalyst HO-1 hemoglobin declines the solution as the courage green vegetable, CO and irons, the former revivification has the very strong anti- to oxidize the ability after become the red vegetable of courage , the latter is a kind of important letter to make the member.

     

    应用推荐稀释比例
    {WB}{1:2000-20000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:50-200}
    {IF}{1:100-500}
    {Flow-Cyt}{1:50-100}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片7
    Sample:
    Lane 1: Mouse Liver tissue lysates
    Lane 2: Mouse Placenta tissue lysates
    Lane 3: Mouse NIH/3T3 cell lysates
    Lane 4: Rat Spleen tissue lysates
    Lane 5: Rat Liver tissue lysates
    Lane 6: Human HeLa cell lysates
    Lane 7: Human 293T cell lysates
    Lane 8: Human HepG2 cell lysates
    Primary: Anti-Heme Oxygenase 1 (bsm-60751R) at 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 32 kDa
    Observed band size: 30 kDa
    产品细节图片8
    25 ug total protein per lane of various lysates (see on figure) probed with Heme Oxygenase 1/HMOX1 monoclonal antibody, unconjugated (bsm-60751R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 8.2 }}] {E Wen. et al. The P2X7-Mediated Mitochondrial ROS as an Emerging Core Target of Tuftsin Nanoparticles in Severe Acute Pancreatitis Therapy via Regulating Mitophagy. ACS APPL MATER INTER. 2025;17(5):7521–7538} {WB} {Mouse}

    [IF={{ 4.7 }}] {Keiichi Hiramoto. et al. Induction of Skin Cancer by Long-Term Blue Light Irradiation. BIOMEDICINES. 2023 Aug;11(8):2321} {IHC} {Mouse}

    [IF={{ 4.6 }}] {Zhiguang Zhang. et al. The Protective Effect of Marsdenia tenacissima against Cisplatin-Induced Nephrotoxicity Mediated by Inhibiting Oxidative Stress, Inflammation, and Apoptosis. MOLECULES. 2023 Jan;28(22):7582} {WB} {Mouse}

    [IF={{ 17.1 }}] {Lei Liu. et al. Myricetin Oligomer Triggers Multi-Receptor Mediated Penetration and Autophagic Restoration of Blood-Brain Barrier for Ischemic Stroke Treatment. ACS NANO. 2024;XXXX(XXX):XXX-XXX} {WB} {Rat}

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    文献支持
    Heme Oxygenase 1 Recombinant Rabbit mAb(bsm-60751R)-50ul/100ul/25ul
    ￥800 - 2500