Sample:
Lane 1: Human Jurkat cell Lysates
Lane 2: Human MOLT4 cell Lysates
Primary: Anti-CD3G (bsm-60390R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20kDa
Observed band size: 25kDa
- ￥800 - 2500
- Bioss
- bsm-60390R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
GBP5 Rabbit pAb, HRP conjugated(bs-7019R-HRP)-100ul
￥2980phospho-Mcl1 (Ser159 + Thr163) Rabbit pAb, APC conjugated(bs-3265R-APC)-100ul
￥2980
KAP3.3 Rabbit pAb(bs-16851R)-50ul/100ul/200ul
￥1180
phospho-FAK (Tyr925) Rabbit pAb, PE conjugated(bs-5540R-PE)-100ul
￥2980
PGC Mouse mAb(bsm-43143M)-50ul/100ul/200ul/200ug
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略：5 种常见类型+关键误区Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60390R |
| 英文名称 | CD3G Recombinant Rabbit mAb |
| 中文名称 | CD3G重组兔单抗 |
| 英文别名 | CD3-GAMMA; CD3g; CD3g antigen gamma polypeptide; CD3g molecule gamma(CD3 TCRcomplex); CD3gmoleculegamma; CD3G_HUMAN; FLJ17620; FLJ17664; FLJ79544; FLJ94613; MGC138597; T cell antigen receptor complex gamma subunit of T3; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 gamma chain; T3G. |
| 产品应用 | WB=1:500-1000, IHC-P=1:500-2000, IHC-F=1:500-2000, ICC/IF=1:50-200, IF=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD3G |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 20 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 |
Immunology > Adaptive Immunity > T Cells > CD |
| 亚细胞定位 | membrane |
| 翻译后修饰 | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8 (PubMed:2470098). Phosphorylated also by PKC; leading to the TCR complex down-regulation (PubMed:8187769). |
| 相似性 | Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain. |
| 功能 | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
| {IHC-P} | {1:500-2000} |
| {IHC-F} | {1:500-2000} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:500-2000} |
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60390R
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60390R
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60390R
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60390R
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:2000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60390R
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:2000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60390R
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
CST 抗体 9 月特惠｜购买任意 CST 抗体，+168 元即可入手 25μl 标签 / 内参抗体
mAb (S-114-13) 裸抗 168 元 S0B0271-25u1 S-Rab® GST Tag Recombinant Rabbit mAb(S-372-19) 裸抗 168 元 S0B1593-25u1 B-tubulin Recombinant Rabbit mAb (HRP Conjugate) (S-R014) 直标抗体 298 元 S0B1648-25u1 FLAG Tag Recombinant Rabbit mAb (HRP Conjugate)(S-589
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
2S5I) Rabbit mAb 抗体 41255S SLK Antibody 抗体 9062S Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb 抗体 S0B0035 PLK1 Recombinant Rabbit mAb (SDT-R062) 抗体 abs580421 α-酮戊二酸单钠盐 小分子化合物 四、苯乙酰化（Phenylacetylation） 2026年6月，中国科学院上海药物研究所谭敏佳/李静雅/徐骏宇团队与华东理工大学叶邦策团队合作，在《Cell
技术资料暂无技术资料 索取技术资料
CD3G Recombinant Rabbit mAb(bsm-60390R)-50ul/100ul/25ul
￥800 - 2500
