Sample:
Lane 1: U-87MG cell lysates
Lane 2: K562 cell lysates
Lane 3: HeLa cell lysates
Lane 4: A431 cell lysates
Primary: Anti-PLOD1 (bsm-51740M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 85 kD
- ￥1180 - 1980
- Bioss
- bsm-51740M
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-51740M |
| 英文名称 | PLOD1 Mouse mAb |
| 中文名称 | 赖氨酸羟化酶1单克隆抗体 |
| 英文别名 | PLOD; PLOD1_HUMAN; 2-oxoglutarate 5-dioxygenase 1; EDS6; LH; LH1; LLH; Lysine hydroxylase; Lysyl hydroxylase 1; Procollagen lysine 1 2 oxoglutarate 5 dioxygenase 1; Procollagen lysine 2 oxoglutarate 5 dioxygenase 1; Procollagen-lysine. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human PLOD1 |
| 亚型 | IgG2b,k |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 33 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Collagen |
| 亚细胞定位 | Rough endoplasmic reticulum membrane. |
| 相似性 | Contains 1 Fe2OG dioxygenase domain. |
| 功能 | Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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PLOD1 Mouse mAb(bsm-51740M)-50ul/100ul
￥1180 - 1980
