Sample:
_x000D_
Lane 1: Mouse Cerebrum tissue lysates
_x000D_
Lane 2: Mouse Cerebellum tissue lysates
_x000D_
Lane 3: Rat Cerebrum tissue lysates
_x000D_
Lane 4: Rat Cerebellum tissue lysates
_x000D_
Lane 5: Human SH-SY5Y cell lysates
_x000D_
Primary: Anti-CELF3 (bs-13830R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 52 kD
_x000D_
Observed band size: 42-55 kD
- ￥1180 - 2800
- Bioss
- bs-13830R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13830R |
| 英文名称 | CELF3 Rabbit pAb |
| 中文名称 | CELF3蛋白抗体 |
| 英文别名 | Bruno-like protein 1; BRUNOL1; CAG repeat protein 4; CAGH4; CELF-3; CELF3; CELF3_HUMAN; CUG-BP-and ETR-3-like factor 3; CUGBP Elav-like family member 3; ELAV-type RNA-binding protein 1; ERDA4; ETR-1; Expanded repeat domain protein CAG/CTG 4; RNA-binding protein BRUNOL-1; TNRC4; Trinucleotide repeat-containing gene 4 protein. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CELF3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing > Splicing |
| 亚细胞定位 | Nucleus. Cytoplasm. |
| 组织特异性 | Expressed in brain. |
| 相似性 | Belongs to the CELF/BRUNOL family. _x000D_ Contains 3 RRM (RNA recognition motif) domains. |
| 功能 | RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
_x000D_ HepG2 (human)cell Lysate at 40 ug
_x000D_ A549 (human)cell Lysate at 40 ug
_x000D_ Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52kD
_x000D_ Observed band size: 57kD
_x000D_
_x000D_ HepG2 (human)cell Lysate at 40 ug
_x000D_ A549 (human)cell Lysate at 40 ug
_x000D_ Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52kD
_x000D_ Observed band size: 57kD
_x000D_
Sample: Lovo (human)cell Lysate at 40 ug
_x000D_ Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52kD
_x000D_ Observed band size: 57kD
_x000D_
_x000D_ Primary: Anti- CELF3(bs-13830R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52kD
_x000D_ Observed band size: 57kD
_x000D_
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[IF=0] Ma P et al. Mass spectrometric analysis of active ingredients in fertilized egg for lipid metabolism. 29 January 2020, PREPRINT (Version 1) available at Research Square. WB chicken
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CELF3 Rabbit pAb(bs-13830R)-50ul/100ul/200ul
￥1180 - 2800
