Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:100
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60253M
- ￥800 - 2500
- Bioss
- bsm-60253M
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60253M |
| 英文名称 | SMARCA5 Recombinant Mouse mAb |
| 中文名称 | SMARCA5单克隆抗体 |
| 英文别名 | SMCA5_HUMAN; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135; EC:3.6.4.-; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; Sucrose nonfermenting protein 2 homolog(hSNF2H); SNF2H; |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 亚型 | IgG2b/Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 理论分子量 | 122 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Remodeling > Swi / Snf |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Ubiquitously expressed. |
| 相似性 | Belongs to the SNF2/RAD54 helicase family. ISWI subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 SANT domains. |
| 功能 | Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50-100} |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MCF-7
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 120 kDa
Observed MW: 120 kDa
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MCF-7
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 120 kDa
Observed MW: 120 kDa
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SMARCA5 Recombinant Mouse mAb(bsm-60253M)-50ul/100ul/25ul
￥800 - 2500
