Sample:
Lane 1: Human K562 cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human 293T cell lysates
Primary: Anti-MIST1 (bsm-34219M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 27 kDa
- ￥1180 - 1980
- Bioss
- bsm-34219M
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-34219M |
| 英文名称 | BHLHA15 Mouse mAb |
| 中文名称 | BHLHA15单克隆抗体 |
| 英文别名 | BHA15_HUMAN; Class A basic helix-loop-helix protein 15; bHLHa15; Class B basic helix-loop-helix protein 8(bHLHb8); Muscle, intestine and stomach expression 1(MIST-1); BHLHB8; MIST1; basic helix-loop-helix family member a15; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig) |
| 抗体来源 | Mouse |
| 免疫原 | Recombinded human BHLHA15 protein |
| 亚型 | IgG2a |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 21 kDa |
| 实际分子量 | 27kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in brain, liver, spleen and skeletal muscle. |
| 功能 | Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in several processes, including cellular response to glucose starvation; endoplasmic reticulum unfolded protein response; and negative regulation of myotube differentiation. Predicted to act upstream of or within several processes, including glucose homeostasis; intracellular distribution of mitochondria; and mitochondrial calcium ion transmembrane transport. Predicted to be part of chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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BHLHA15 Mouse mAb(bsm-34219M)-50ul/100ul
￥1180 - 1980
