- ￥585 - 1053
- Biorbyt
- 英国
- orb1736815
- 2026年08月07日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
12 months from date of receipt.
- 英文名：
PCR Master Mix(Red)
- 库存：
99
- 供应商：
Biorbyt
- 规格：
1 ml/5 ml
| 规格： | 1 ml | 产品价格： | ￥585.0 |
|---|---|---|---|
| 规格： | 5 ml | 产品价格： | ￥1053.0 |
描述: . . PCR Master Mix is a ready-to-use conventional PCR master mix solution containing Taq DNA Polymerase, dNTP mix, MgCl2, and optimized buffer system. The amplification can be performed by adding primers and templates during the reaction, which greatly simplifies the operation steps of the experiment. The product contains cresol red, and the PCR product can be directly electrophoresed. This product contains excellent stabilizer and can be stored for 3 months at 4°C. PCR products have 3'-dA overhangs for easy cloning into T-vectors
研究领域: Stem Cell & Developmental Biology; Molecular Biology
备注: For research use only.
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文献和实验-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
of source DNA material • It does not necessarily require the use of radioisotopes or toxic chemicals • It involves preparing the sample DNA and a master mix with primers[Primer: A short DNA or RNA fragment annealed to a singled-stranded DNA
a proofreading polymerase and buffers included in the kit Triple Master Mix (Eppendorf, Hamburg, Germany). The standard reaction conditions were 96°C for 3 min, followed by 30 cycles with 96°C, 45 s, 57°C 45 s, 72°C, 1�3 min, depending on the size of the product
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