- ￥3510 - 9945
- Biorbyt
- 英国
- orb1736812
- 2026年08月07日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
12 months from date of receipt.
- 英文名：
SYBR Green PCR SuperMix(Low ROX Premixed)
- 库存：
99
- 供应商：
Biorbyt
- 规格：
5 ml/25 ml
| 规格： | 5 ml | 产品价格： | ￥3510.0 |
|---|---|---|---|
| 规格： | 25 ml | 产品价格： | ￥9945.0 |
描述: 1.Quickly get results,saving up to 50% of the time |2.Optimized ready-to-use master mix for rapid PCR reactions |3.Accurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability |4.Balanced K+ and NH4+ ion ratios, as well as stand-alone ROX Reference Dye packaging for all real-time PCR instruments. . SYBR Green PCR SuperMix is an optimized 2x real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green fluorescent dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization
研究领域: Molecular Biology
备注: For research use only.
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文献和实验SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL
1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度，需要摸索，从200nM到700nm等，设置不同浓度。3、每个引物浓度，从well 1到12设置4个样本，阳性cDNA 1和2，NTC以及H2O，做triplicate。4、若用ABI7000或7700，所有的PCR条件可设置成一致的，减少麻烦，当然，引物
in SYBR green Ⅰreal2time RT-PCR. In addition , the 42step program may be used for quantifying low abundance RNA ,while the 32step program may not . The primer pair with low Tm should be preferably selected for the efficient elimination of the effect of PDs
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