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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6-12个月
- 英文名:
S-30-Hydroxygambogic acid
- 库存:
99
- 供应商:
Biorbyt
- CAS号:
881027-36-7
- 规格:
50 mg/5 mg
| 规格: | 50 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | 询价 |
S-30-Hydroxygambogic acid
产品名:S-30-Hydroxygambogic acid
货号:orb1943532
产品中文名:S-30-Hydroxygambogic acid
产品详情链接:https://www.biorbyt.com/s-30-hydroxygambogic-acid-orb1943532.html
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文献和实验Preparation and Use of E. coli S-30 Extracts
to direct the synthesis of proteins, which could be directly characterized. It is these characteristics of the E. coli -coupled transcription/translation system (S-30) that are still useful for a variety of applications. The in vitro system, first described
DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts
for at least 30 min at 37�C before gene products are examined. The following method is essentially as described by Zubay (1 ), but with minor modifications as described by Valentin-Hansen et al. (2 ).
物的容器中,将捣杆垂直插入匀浆管,上下转动研磨数十次(6~8分钟),使细胞破碎。 机械匀浆:将样本(含匀浆介质)装入EP管,用机械匀浆机,在低温条件下,60 Hz,90 s研磨,使细胞破碎。 超声破碎:用超声波发生器以振幅14 μm超声处理30 s,使细胞破碎;或者用超声细胞破碎仪,300 W,3~5 s/次,间隙30 s,重复4~5次。 反复冻融:用PBS溶液或双蒸水重悬细胞,再将细胞悬液进行“冷冻-融化-冷冻”循环,重复3次左右。此法会使某些酶活力受影响,因此检测细胞样本的酶活力时不推荐使用反复
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