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- Biorbyt
- 英国
- orb1943033
- 2026年08月06日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
12 months from date of receipt.
- 英文名：
Myosin light chain kinase fragment 11-19 amide
- 库存：
99
- 供应商：
Biorbyt
- 规格：
5 mg/50 mg
| 规格： | 5 mg | 产品价格： | ￥0.0 |
|---|---|---|---|
| 规格： | 50 mg | 产品价格： | ￥0.0 |
描述: MLCK(11-19) amide is a peptide inhibitor that specifically targets myosin light chain kinase, blocking its activity and inhibiting Ca2+ influx triggered by hypotonic stress. It is utilized in biochemical assays and cellular research, including studies on cervical cancer cell lines, to investigate calcium signaling and cytoskeletal regulation.
CAS号: 119386-39-9
分子式: C40H78N18O11
分子量: 987.16
SMILES: [C@@H](NC([C@@H](NC([C@H](CCCCN)N)=O)CCCCN)=O)(C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N)=O)CO)=O)[C@@H](C)O)=O)C)=O)CCCNC(=N)N)=O)C)=O)C)=O)CCCNC(=N)N
研究领域: Immunology & Inflammation; Musculoskeletal & Connective Tissue Research
保存条件: -20°C
备注: For research use only.
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
Image Figure 20.9.6 Kinetic plots of amide deuteration after data analysis to correct for back exchange and residual side‐chain deuteration
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