AMI-1, free acid，orb1905872，Bi

Protein Charge Determination

of determining protein valence entails the calculation of net charge from amino acid sequence/composition. However, the inaccuracy of that approach was recognized long before the advent of the protein data banks and computer programs to facilitate its adoption

化学发光免疫测定方法的研究进展

睾酮的增高常提示肿瘤的可能性。黄岚等人[8]的测定结果显示ELISA法测定的灵敏度为0.196ng／ml，CLEIA法检测的灵敏度为0.063ng／mL，二法的相关系数r=0．971，所以得用CLEIA法代替RIA法及时准确的检测血清中睾酮的含量对临床有很重要意义。Del等人[9]测定绝经后期骨质疏松患者的尿中free-deoxypyfidino-line（DPD），分别采用ELISA法、CLEIA法、HPIE（高效液相色谱法）法三种方法测定，结果显示三种方法中ELISA法和CLEIA法是最好的辨别骨质