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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6 months from date of receipt.
- 英文名:
Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda)
- 库存:
99
- 供应商:
Biorbyt
- 规格:
20 ug/100 ug/1 mg
| 规格: | 20 ug | 产品价格: | ¥5265.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥9360.0 |
| 规格: | 1 mg | 产品价格: | ¥30537.0 |
描述: This Recombinant Enterobacteria phage T4 ATP-dependent DNA helicase dda (dda) spans the amino acid sequence from region 1-439aa. Purity: Greater than 85% as determined by SDS-PAGE.
形态: Liquid or Lyophilized powder
应用说明: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
生物来源: Enterobacteria phage T4 (Bacteriophage T4)
表达区域: 1-439aa
分子量: 57.3 kDa
防腐剂: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
蛋白长度: Full Length
蛋白序列: MTFDDLTEGQKNAFNIVMKAIKEKKHHVTINGPAGTGKTTLTKFIIEALISTGGTGIILAAPTHAAKKILSKLSGKEASTIHSILKINPVTYEENVLFEQKEVPDLAKCRVLICDEVSMYDRKLFKILLSTIPPWCTIIGIGDNKQIRPVEPGENTAYISPFFTHKDFYQCELTEVKRSNAPIIDVATDVRNGKWNYDKVVDGHGVRGFTGDTALRDFMVNYFSIVKSLDDLFENRVMAFTNKSVDKLNSIIRKKIFETDKDFIVGEIIVMQEPLFKTYKIDGKPVSEIIFNNGQLVRIIEAEYTSTFVKARGVPGEYLIRHWDLTVETYGDDEYYREKIKIISSDEELYKFNLFLAKTAETYKNWNKGGKAPWSDFWDAKSQFSKVKALPASTFHKAQGMSVDRAFIYTPCIHYADVELAQQLLYVGVTRGRYDVFYV
纯度: Greater than 85% as determined by SDS-PAGE.
来源: E.coli
标签: N-terminal 10xHis-tagged and C-terminal Myc-tagged
研究领域: Infectious Disease & Virology
UniProt ID: P32270
保存条件: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
备注: For research use only.

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文献和实验DNA Ligation and Escherichia coli Transformation
DNA ligase, which requires Mg2+ and NAD+ as cofactors and is less commonly used, and T4 DNA ligase produced by the E. coli phage T4, which requires Mg2+ and ATP as cofactors. The in vivo function of both these enzymes is to repair nicks in double
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
同源重组(Homologus Recombination) 是指发生在姐妹染色单体(sister chromatin) 之间或同一染色体上含有同源序列的DNA分子之间或分子之内的重新组合。同源重组需要一系列的蛋白质催化,如原核生物细胞内的RecA、RecBCD、RecF、RecO、RecR等;以及真核生物细胞内的Rad51、Mre11-Rad50等等。 在人类细胞中,同源重组由Rad51介导,在有ATP的条件下,Rad51聚合在单链DNA上,形成一个核蛋白
segments, where phosphodiester bonds are formed between adjacent 3′-OH and 5′-phosphate termini of DNA and RNA . The most widely used of such enzymes is T4 DNA Ligase. This enzyme can join DNA fragments having cohesive or blunt ends. It requires ATP
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