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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
31.2-2000pg/ml
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, cell culture supernatant and other biological samples
- 灵敏度:
18.75pg/ml
- 规格:
48 T
产品别名:IGHV3-9|Immunoglobulin heavy variable 3-9|Ig heavy chain V-III region DOB
实验时长:4 hours
UniProt ID:P01782
靶点:IGHV3-9
保存说明:2 - 8 °C for 12 months
Note:For research use only.
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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
The quantification of human immunoglobulin G (IgG) and recombinant derivatives is required for many applications in research and diagnostics. Particularly in research, robust and simple protocols are necessary to determine the concentration
一篇文献中的ELISA方法如下:Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
结果的对照,因此对照品,特别是阳性对照品的基本组成应尽量与检测标本的组成相一致。以人血清为标本的测定,对照品最好也为人血清,因为正常人血清在各种ELISA模式中可产生不同程度的本底。由于大量正常人血甭较难得到,国外试剂盒中的对照品多以复钙人血浆(recalcified human plasma)为原料,即在血浆中加入钙离子,使其中的纤维蛋白质凝固,除去凝块后所得的液体,其组成与血清相似。阴性对照品须先行检测,确定其中不含待测物质。例如HBsAg检测的阴性对照品中不可含HBsAg,最好抗HBs也是阴性
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