- ￥3510
- biorbyt
- 0.78-50ng/mL
- 0.62 ng/mL
- orb1807445
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.78-50ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, saliva, colostrum, Gastrointestinal secretions
- 灵敏度：
0.62 ng/mL
- 规格：
48 T
产品别名：IgA
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgA. You can calculate the concentration of Human IgA in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P01876
靶点：IgA
Note：For research use only.
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文献和实验Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin
Human immunoglobulin A (IgA) is found in serum and secretions in several different forms (1 ), Serum IgA is produced by the bone marrow and is pre-dominately monomeric (M r = 160 kDa
Immunoglobulin Class Switching: In Vitro Induction and Analysis
During an immune response, B lymphocytes can switch expression of immunoglobulin (Ig) class (isotype) from IgM to IgG, IgE, or IgA. This Ig class switch is based on a deoxyribonucleic acid (DNA) recombination event that results in an exchange
抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类，即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链（L）和两个重链（H）的单体组成。Ig 的轻链是相同的，有 κ（kappa）和 λ（Lambda）两种型别。五类 Ig 的重链结构不同，这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ（gamma）链和 μ（mu ）链。 重链和轻链的 N 端的氨基酸排列顺序
