- ￥3627 - 5031
- Biorbyt
- 0.31-20ng/mL
- 0.19 ng/mL
- orb1807931
- 英国
- 2026年08月04日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
Biorbyt
- 检测范围：
0.31-20ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.19 ng/mL
- 规格：
48 T/96 T
| 规格： | 48 T | 产品价格： | ￥3627.0 |
|---|---|---|---|
| 规格： | 96 T | 产品价格： | ￥5031.0 |
描述: Mouse Glucose Regulated Protein 78 (GRP78) ELISA Kit
别名: Hspa5
靶点: GRP78
应用说明: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRP78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRP78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRP78. You can calculate the concentration of Mouse GRP78 in the samples by comparing the OD of the samples to the standard curve.
检测时间: 3.5H
标准品: 20ng/mL
研究领域: Pharmacology & Drug Discovery; Cell Biology
UniProt ID: P20029
备注: For research use only.
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文献和实验化。BAPTA的pKa3为5.47，pKa4为6.36。这个特点显示了去质子化的步骤并不包含在Ca的螯合步骤中。由于不受质子的干扰，它的螯合率比EGTA要高很多。BAPTA-AM是BAPTA的乙酰甲酯衍生物，通过AM法，能被轻易负载到细胞中。BAPTA-AM对于细胞内钙离子的监控是非常有用的。 kaige88 葡萄糖调节蛋白（glucose regulated proteins, GRPs）是内质网中一类重要的驻留蛋白，包括GRP78和GRP94。 GRP
with chaperones in these cells is of interest. Furthermore, Pb and Cu bind strongly to the molecular chaperone heat shock 70 kDa protein Hspa5, also known as glucose-regulated protein 78 (Grp78) or immunoglobulin-binding protein (BiP). This chapter describes
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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