- ￥3770
- biorbyt
- 0.31-20ng/mL
- 0.19 ng/mL
- orb1807931
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.31-20ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.19 ng/mL
- 规格：
48 T
产品别名：Hspa5
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRP78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRP78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRP78. You can calculate the concentration of Mouse GRP78 in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P20029
靶点：GRP78
Note：For research use only.
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