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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Biorbyt
- 检测范围:
15.63-1000pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
5.47 pg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥3627.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥5031.0 |
描述: Human Interleukin 37 (IL-37) ELISA Kit
别名: IL37 , FIL1, FIL1(ZETA) , FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-37, IL1F7, IL1H4, IL1RP1
靶点: IL-37
应用说明: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-37. You can calculate the concentration of Human IL-37 in the samples by comparing the OD of the samples to the standard curve.
检测时间: 3.5H
标准品: 1000pg/mL
研究领域: Immunology & Inflammation
UniProt ID: Q9NZH6
备注: For research use only.

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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
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调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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